SEPLITE® CM2100/CM2100(S) for the preparation and purification of small biological molecules

SEPLITE® CM2100/CM2100(S) is a special filler with divinylbenzene polymer backbone and linear styrene as the functional base. It provides excellent kinetic performance for the preparation and purification of small biological molecules. The resin provides balanced pressure flow characteristics and true chromatographic separation capabilities, and it can also be successfully applied to the simulated moving bed (SMB) separation of various small biomolecules. This resin is often used in the preparation and industrial purification of hydrophilic small molecules instead of bonded silica gel.

SEPLITE® CM2100/CM2100(S) for the preparation and purification of small biological molecules Physical and Chemical Characteristics:

SEPLITE® CM2100/CM2100(S) for the preparation and purification of small biological molecules Performance characteristics:

1. Suitable for a wide range of applications - suitable for the purification of most small molecules (the most successful polydisperse reversed-phase packing developed so far);

2. Large loading capacity, 10-20% larger than similar resin loading on the market (compared to main products).

3. Lower back pressure compared with international main products, higher temperature post-processing technology eliminates product swelling, making the product back pressure lower;

4. Strong capture ability. Compared with the above-mentioned reversed-phase fillers, this product has stronger adsorption and capture ability for small hydrophilic molecules. It is suitable for the separation of small molecules with strong hydrophilicity (≤1500Da).

SEPLITE® CM2100/CM2100(S) for the preparation and purification of small biological molecules Product instructions:

1.Pretreatment. The resin is stored in a 20% ethanol aqueous solution during transportation. Please wash it with distilled water before using; soak it with a solvent of suitable concentration for 16-24h before loading the column. In order to prevent the resin from swelling during column packing and causing column pressure changes (e.g. the extract is 30% acetonitrile aqueous solution, then use 30% acetonitrile for soaking). Please remove floating particles before packing to prevent the column from blocking the packing).

2.The resin withstand voltage range <50bar.

3.Load the sample. (In the first test, the sample amount can be appropriately reduced, and then the sample amount can be increased according to the retention time and peak shape of the target).

4.Cleaning. (Gradient elution can be performed directly in the first test. Afterwards, select the appropriate cleaning solution concentration according to the retention time of the target. As in the above example, use 50% acetonitrile aqueous solution for cleaning).

5.Elution. (Gradient elution can be performed for the first test, and isocratic elution is recommended for scale. As in the above example, 75% acetonitrile aqueous solution is used for elution).

6.CIP cleaning and post-treatment. Generally, use organic solvents, high-concentration acids and alkalis to clean the resin. After cleaning, wash with a large amount of distilled water until it is neutral, and keep it for the next use. If you will not use it for a long time, please use 20% ethanol to store the resin).