Chromatographic separation is an effective method for separating components in complex mixtures, which is based on different partition coefficients of different substances in stationary phase and mobile phase. When the two phases move in relative motion, these substances move together with the mobile phase and are distributed repeatedly between the two phases. Thus, different substances are eluted at different places and separation is achieved.

Seplite® MonojetTM CM1850 uniform particle strong acid cation exchange resinproduced by advanced "jet granulation" technology after repeated tests and demonstrations. By using the special structural design and functional group setting of the resin, the binding force between the resin and glucose and fructose can be differentiated, and the efficient separation of glucose and fructose can be achieved through the elution of spreading agent according to the degree of difficulty of separation.

Chromatography technology is an important technology in the field of modern separation.

Chromatographic separation technology is a kind of separation method widely used in the separation and purification of complex mixtures.

In the separation and purification, the column is required to have high loading capacity, easy operation and long service life.

When selecting a buffer, pH and ionic strength are two key factors that affect not only the separation of the target product from the impurities, but also the yield of the product. The pH chosen depends on the isoelectric point, stability and solubility of the target product, not only to make the separated material an ion that can be exchanged, but also to maintain its high activity. The pK value of the ion exchanger should also be considered.

In the industrial products of ion exchangers, a small number of organic oligomers and some inorganic impurities are often contained.

When the chromatographic medium is exhausted, it should be eluted. The basic principle is to desorb the target product by a more active ion or group. Different sorbents are different in their activity. Therefore, a suitable eluent should be selected to elute the protein from chromatography media.