The heteroatoms on the chelating resin can form a coordination bond with a metal ion by a pair of lone pairs of electrons, forming a stable structure similar to a small molecule chelate. The polymer with chelation function needs to meet the requirements of two aspects, firstly, it needs to contain a coordination group, and secondly, the coordination group is arranged on the polymer backbone to ensure the space configuration requirements of the chelation process.

A chelating resin is a cross-linking functional polymer material capable of forming a multi-coordination complex with a metal ion.

Boron, Fluorine ,Magnesium, Calcium, Vanadium ,Chromium, Manganese , Iron, Cobalt.

Chromatographic separation technology is a kind of separation method widely used in the separation and purification of complex mixtures.

In the separation and purification, the column is required to have high loading capacity, easy operation and long service life.

When selecting a buffer, pH and ionic strength are two key factors that affect not only the separation of the target product from the impurities, but also the yield of the product. The pH chosen depends on the isoelectric point, stability and solubility of the target product, not only to make the separated material an ion that can be exchanged, but also to maintain its high activity. The pK value of the ion exchanger should also be considered.

In the industrial products of ion exchangers, a small number of organic oligomers and some inorganic impurities are often contained.

When the chromatographic medium is exhausted, it should be eluted. The basic principle is to desorb the target product by a more active ion or group. Different sorbents are different in their activity. Therefore, a suitable eluent should be selected to elute the protein from chromatography media.