Case Study

When selecting a buffer, pH and ionic strength are two key factors that affect not only the separation of the target product from the impurities, but also the yield of the product. The pH chosen depends on the isoelectric point, stability and solubility of the target product, not only to make the separated material an ion that can be exchanged, but also to maintain its high activity. The pK value of the ion exchanger should also be considered.

In the industrial products of ion exchangers, a small number of organic oligomers and some inorganic impurities are often contained.

When the chromatographic medium is exhausted, it should be eluted. The basic principle is to desorb the target product by a more active ion or group. Different sorbents are different in their activity. Therefore, a suitable eluent should be selected to elute the protein from chromatography media.

When the operation is carried out for a certain period of time, the resin bed will be divided into a saturated resin zone, a mass transfer zone, and a fresh resin zone. As the run time increases, the mass transfer zone gradually decreases until the bottom of the bed is penetrated by the mass transfer zone and the entire resin layer fails.

The functional group of the resin determines the properties and uses of the resin.

The whole section of resin in the original fixed bed is divided into several sections.

The ion exchange is a technique for separating using an exchangeable group in an ion exchanger and a difference in ion exchange capacity between various ions in a solution, and is a method of solid-liquid separation.

The unique properties of ion exchange resins are due to their special internal structure, which is the combination of physical and chemical action.