A chelating resin is a cross-linking functional polymer material capable of forming a multi-coordination complex with a metal ion.

Boron, Fluorine ,Magnesium, Calcium, Vanadium ,Chromium, Manganese , Iron, Cobalt.

Chromatographic separation technology is a kind of separation method widely used in the separation and purification of complex mixtures.

In the separation and purification, the column is required to have high loading capacity, easy operation and long service life.

When selecting a buffer, pH and ionic strength are two key factors that affect not only the separation of the target product from the impurities, but also the yield of the product. The pH chosen depends on the isoelectric point, stability and solubility of the target product, not only to make the separated material an ion that can be exchanged, but also to maintain its high activity. The pK value of the ion exchanger should also be considered.

In the industrial products of ion exchangers, a small number of organic oligomers and some inorganic impurities are often contained.

When the chromatographic medium is exhausted, it should be eluted. The basic principle is to desorb the target product by a more active ion or group. Different sorbents are different in their activity. Therefore, a suitable eluent should be selected to elute the protein from chromatography media.

When the operation is carried out for a certain period of time, the resin bed will be divided into a saturated resin zone, a mass transfer zone, and a fresh resin zone. As the run time increases, the mass transfer zone gradually decreases until the bottom of the bed is penetrated by the mass transfer zone and the entire resin layer fails.