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05-07-2019

Chromatographic Separation

Chromatographic separation technology is a kind of separation method widely used in the separation and purification of complex mixtures.
05-07-2019

Selection of Ion Exchange Chromatography Media

In the separation and purification, the column is required to have high loading capacity, easy operation and long service life.
05-07-2019

Buffer Solution

When selecting a buffer, pH and ionic strength are two key factors that affect not only the separation of the target product from the impurities, but also the yield of the product. The pH chosen depends on the isoelectric point, stability and solubility of the target product, not only to make the separated material an ion that can be exchanged, but also to maintain its high activity. The pK value of the ion exchanger should also be considered.
05-07-2019

Pretreatment of Chromatography Media

In the industrial products of ion exchangers, a small number of organic oligomers and some inorganic impurities are often contained.
05-07-2019

Elution & Regeneration

When the chromatographic medium is exhausted, it should be eluted. The basic principle is to desorb the target product by a more active ion or group. Different sorbents are different in their activity. Therefore, a suitable eluent should be selected to elute the protein from chromatography media.
05-07-2019

How Does Ion Exchange Column Work?

When the operation is carried out for a certain period of time, the resin bed will be divided into a saturated resin zone, a mass transfer zone, and a fresh resin zone. As the run time increases, the mass transfer zone gradually decreases until the bottom of the bed is penetrated by the mass transfer zone and the entire resin layer fails.
05-07-2019

Basic Type of Ion Exchange Resin

The functional group of the resin determines the properties and uses of the resin.
05-07-2019

What is Simultaneous Moving Bed?

The whole section of resin in the original fixed bed is divided into several sections.
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